FastQCFastQC Report
Thu 26 May 2016
SRR1781459_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781459_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367954
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT16909245.954657375650214No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG117633.1968669996793078No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC70971.928773705408828No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC24550.6672029655880899No Hit
ATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCTT19570.5318599607559641Illumina PCR Primer Index 7 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17440.4739722900145127No Hit
CTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC15540.42233540062072983No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG10520.28590530338031384No Hit
CTTTACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC7060.1918718100632144No Hit
TTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC6490.17638074324507955No Hit
CCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG5570.15137761785440573No Hit
CTTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG5480.14893165993575283No Hit
CTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT5380.1462139289150274No Hit
ATTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT5200.14132201307772166No Hit
TATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCT4800.13045108899482No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGACATCTCGTATGCCGT4550.12365676144300645No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCACGTATGCCGT4020.10925278703316176No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC3980.10816569462487159No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGACGT3720.1010995939709855No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAA150.002223082870.0048932
AAACGCT150.002223082870.0048929
CAAATCG207.918725E-570.0048936
ACTCGAA252.8359755E-670.0048938
ATCGAAT150.002223082870.0048939
ACCGAGA207.924058E-569.9953813
GCACGAT207.924058E-569.9953823
CACGATG207.924058E-569.9953824
GCCGACA150.002224281469.9953846
CGTAATA252.8382674E-669.9953848
CGACTAC150.002224281469.9953848
AGGGGGG191500.067.6561170
ACGGACA1300.064.61989631
CGGACAT1200.064.1711532
AAGGGGG207400.062.43560869
ATCGTAT850.061.7690239
AACGGAC1500.060.67090630
CTACGAG750.060.66266312
TCGTAAG1850.060.54476540
ATCTACG700.059.9960410
Produced by FastQC (version 0.11.5)