FastQCFastQC Report
Thu 26 May 2016
SRR1781458_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781458_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220154
Sequences flagged as poor quality0
Sequence length76
%GC83

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14021263.68814557082769No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG207.922799E-569.9849922
CGAGGGT150.002226404169.96908625
TGCCGGC452.6850103E-854.4575419
GCCGGAT200.00692551952.53652654
TCGCCGG200.00693799752.51262752
ATAATTA200.00693799752.51262752
TGTGGCG200.00693799752.51262711
TCGGGCG200.00693799752.51262711
CGTGTGG200.006944242452.500689
GCGGATC200.006944242452.500686
GCGTGTG200.006944242452.500688
GACGAAG200.00695049252.48874723
GCGGTAC200.006956745452.4768145
CGGGGGT407.4792297E-752.4768146
GCTGTAT200.006963003452.4648861
CCTAGAC200.00696926552.4529652
ACTAGAC200.00696926552.45296538
CTGCCGG451.6741233E-646.68850718
TGGACTG305.875187E-446.6566625
GCGGTGT305.888373E-446.63545233
Produced by FastQC (version 0.11.5)