FastQCFastQC Report
Thu 26 May 2016
SRR1781458_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781458_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220154
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT10459047.50765373329578No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG102064.635845817018995No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC42031.909118162740627No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC22601.0265541393751647No Hit
ATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCTT17870.8117045340988581TruSeq Adapter, Index 11 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC15240.692242702835288No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAGT10830.4919283774085413No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG8930.4056251533017797No Hit
TTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC6960.3161423367279268No Hit
CTTTACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC6090.276624544636936No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATCTCGTATGCCGTCT5970.271173814693351RNA PCR Primer, Index 38 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGACGT5450.24755398493781625No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCCGT5010.22756797514467145No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG4630.2103073303233191No Hit
TATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCT4230.1921382305113693RNA PCR Primer, Index 11 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC4130.18759595555838185No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG4090.18577904557718689No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTAAGCCGT4040.18350790810069315No Hit
ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT3550.16125076083105463No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTAAATCTCGTATGCCGT3460.15716271337336593No Hit
CTTATACACATCTCCGAGCCAACGAGACTCAGCTATATCTCGTATGCCGT3190.1448985710002998No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCAGT2700.12264142373066127No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTAAGCAGT2580.11719069378707632No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTAATCTCGTATGCCGTC2540.11537378380588134RNA PCR Primer, Index 29 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATAG150.002221078870.0113636
TAACGTA207.90805E-570.0113538
TGCTTAA150.00222308169.9954655
ATATGTC150.00222308169.9954650
CGTCTAA150.00222308169.9954648
ATCAACG150.00222308169.9954610
TACGAGC600.069.9954613
AACGAGC252.8344566E-669.9954613
ACTAAGA150.00222308169.9954627
CGAGCAA252.8344566E-669.9954615
TAGTGAC150.00222308169.9954640
ACGAGCA150.00222308169.9954614
AATACCT150.00222308169.9954644
TCAACGA150.00222308169.9954611
AAGACGT207.916955E-569.9954544
CGACTTA207.916955E-569.9954548
AGGGGGG126700.065.9625770
CTACTGC650.064.6111951
TCTACGA650.064.6111911
ACGTATG650.064.6111940
Produced by FastQC (version 0.11.5)