FastQCFastQC Report
Thu 26 May 2016
SRR1781457_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781457_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199035
Sequences flagged as poor quality0
Sequence length76
%GC80

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12193261.261587158037536No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG301.0182703E-770.01636522
TTTTGTG207.903349E-570.01636518
GATGTGA207.9428646E-569.945931
CTCGGTG800.056.84536444
TCTCGGT800.056.84536443
CCGGGGG252.383453E-456.01309221
GGCGACG252.383453E-456.01309220
TTTCGGA252.3923647E-455.97081837
TCGGTGG900.054.41607345
CGTATCA653.6379788E-1253.87230356
GCCGTAT653.6379788E-1253.87230354
AGTCGGG200.006930292652.52549762
CCGTATC608.367351E-1152.52549455
TGGCGGC200.006937199352.5122719
TGGCGCT200.006937199352.5122713
GCTGCGG200.006937199352.5122717
ACGCTGG200.006937199352.5122715
GACGAGC200.006937199352.5122723
ATTCGAT200.006944110652.49905429
TTCGATG200.006944110652.49905430
Produced by FastQC (version 0.11.5)