FastQCFastQC Report
Thu 26 May 2016
SRR1781457_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781457_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199035
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT9382247.13844298741427No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG94484.746903810887533No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC42532.1368101087748386No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC19750.992287788579898No Hit
ATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCTT16680.8380435601778582TruSeq Adapter, Index 9 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC12210.6134599442309142No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG7350.36928178461074684No Hit
CTTTACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC4830.24267088702991935No Hit
TTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC4580.23011028211118648No Hit
TATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCT4220.2120230110282111No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG3690.18539452860049738No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGACGT3600.18087271082975356No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG3550.17836058984600697No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCAGT3410.17132665109151657No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATATCGTATGCCGT3380.1698193785012686No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC3210.16127816715653026No Hit
ATTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT3080.15474665259878917No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAATCGGATCTCGTATGCCGT2950.14821513804104805No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTAGGATCTCGTATGCCGT2700.13565453312231518No Hit
CTTATACACATCTCCGAGCCAACGAGACGTATTCGGATCTCGTATGCCGT2370.11907453462958777No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGATCTCGTATGCCGTC2260.1135478684653453RNA PCR Primer, Index 42 (95% over 21bp)
CTTATACACATCTCAGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT2160.10852362649785213No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT2120.10651392971085488No Hit
TCTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT2010.10098726354661242No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGAA252.8252362E-670.0296838
TCGATCT301.0169424E-770.0296733
TTCGATC301.0169424E-770.0296732
TGACGAC207.905782E-570.0120745
TATTCGA301.018725E-770.0120730
AATTCGG455.456968E-1270.0120730
GAATTCG500.070.0120729
TAAGCAG207.905782E-570.0120743
GTTATTC150.002222889869.994483
CGTATTA750.069.9944728
GACGACT207.915634E-569.9944746
CCAGACG150.00222510769.9768823
GCTTCTT150.00222510769.9768825
GACTACT207.925497E-569.9768849
AGGGGGG108150.067.0173670
GAGACGA1050.066.6446524
TCGGAAC750.065.3610233
TACTGCT750.065.3281752
CCGTCTA700.064.99486547
ACTCGTA600.064.1938738
Produced by FastQC (version 0.11.5)