FastQCFastQC Report
Thu 26 May 2016
SRR1781456_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781456_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225602
Sequences flagged as poor quality0
Sequence length76
%GC85

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14802065.61112046879018No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTA500.070.037159
TTGACAA150.002219863770.02154519
CCGACGG353.6870915E-970.02154521
CGCGTCT150.002219863770.0215457
ACATTCG150.00222573869.97491527
ATTCGAT150.00222573869.97491529
GTCGGTG150.00222573869.97491544
GTAGGTG252.8433642E-669.9593938
GGTAGGT207.937593E-569.9593937
TGTGCGC252.847115E-669.943863
TACACGT150.002229660569.943865
CGGATAC150.002233588169.912841
CAGTATA150.002233588169.912841
CCGTATC401.0575604E-861.28246355
GCCGTAT401.0593794E-861.26885254
CGTATCA509.713403E-1056.02968256
CGGCGAC252.3832677E-456.0172422
CCGGCGA252.3832677E-456.0172421
GCGCGTC252.3885055E-455.9923636
CATTCGA252.3911278E-455.97993528
Produced by FastQC (version 0.11.5)