FastQCFastQC Report
Thu 26 May 2016
SRR1781456_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781456_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225602
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT11432950.6772989601156No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG111924.960948927757733No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC50062.218951959645748No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC21820.9671900071807874No Hit
ATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCTT16500.7313764948892296TruSeq Adapter, Index 2 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC13240.586874229838388No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG9480.42020904069999376No Hit
TTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC6320.28013936046666255No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGATCTCGTATGCCGTC6170.2734904832403968No Hit
CTTTACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC5810.2575331778973591No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGACGT4750.21054777883174797No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG4690.20788822794124165No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCAGT4410.19547699045221228No Hit
TATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCT4280.18971463018944867No Hit
ATTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT4120.1826224944814319No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG4090.18129271903617875No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATATCGTATGCCGT4070.18040620207267666No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC3790.16799496458364732No Hit
CTTATACACATCTCCGAGCCAACGAGACGGGAATGAATCTCGTATGCCGT3680.16311912128438577No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAAAGAATCTCGTATGCCGT3090.13696687086107392No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT2540.11258765436476627No Hit
CTTATACACATCTCAGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT2370.10505226017499844No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAAC900.070.032391
CCTATGC150.002218504770.0323941
CGAAAGC252.8258128E-670.0323841
GGCAATG207.905392E-570.0168529
ACGCATC207.9140824E-570.001336
AACTTTC150.002222411870.001335
AAAAGCG207.9140824E-570.0013367
TTATACG207.9140824E-570.001332
TACGCAT207.9140824E-570.001335
ACGTCTA207.9140824E-570.0013347
ACGTCAT150.002222411870.0013347
CGACTTA150.002222411870.0013348
TATACGC207.9140824E-570.001333
TATAAGG150.002222411870.001332
AAAGCGG150.002222411870.0013368
CGCATCT207.92278E-569.985817
CGCGAAA207.92278E-569.9858128
GTCTCCG150.002226324369.9703110
ATGTCCG150.002226324369.9703110
GTCCGAG150.002226324369.9703112
Produced by FastQC (version 0.11.5)