FastQCFastQC Report
Thu 26 May 2016
SRR1781455_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781455_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247092
Sequences flagged as poor quality0
Sequence length76
%GC76

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12632051.12265876677513No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGG252.8345803E-669.9991821
CGGTGTA150.00222471869.98501634
TTGTGCG150.002228294769.956682
TTATGCG150.002228294769.956682
CGCGTCT352.970737E-759.987167
TGTGCGC308.38137E-658.309043
CGTGTCA200.006934799752.52066856
CGTCTGG407.4608033E-752.499399
TTCGACT200.0069514952.48875834
TGTCGGG200.0069514952.48875843
GCGCGTC352.0853096E-549.98936
TGACGCG305.87072E-446.6661313
TGTGACG305.87072E-446.6661311
GACGCGG305.87072E-446.6661314
TGCGCGT451.6828599E-646.6566775
GTATCAT802.910383E-1143.76722357
CCGTATC659.95351E-943.0851555
GCGTCTG503.4748682E-641.9910058
CGTATCA701.9112122E-840.0157556
GTGGCCG350.001253925539.9995451
Produced by FastQC (version 0.11.5)