FastQCFastQC Report
Thu 26 May 2016
SRR1781455_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781455_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247092
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT9271437.52205656192835Illumina PCR Primer Index 6 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG93663.7904909912097517No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC39551.6006183931491103No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC21560.8725494957343822No Hit
ATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCTT21260.8604082689848316Illumina PCR Primer Index 6 (95% over 24bp)
CTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC16140.6531979991258317Illumina PCR Primer Index 6 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG9270.3751639065611189No Hit
TTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC6850.27722467744807605Illumina PCR Primer Index 6 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC6410.2594175448820682Illumina PCR Primer Index 6 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCCAATATCTCGTATGCCGT5730.2318974309164198TruSeq Adapter, Index 6 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCAGT5080.20559143962572646No Hit
TATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCT4750.1922360902012206Illumina PCR Primer Index 6 (95% over 23bp)
TCTTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC4710.19061725996794718No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG4630.1873795995014003No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGACGT4520.18292781635989835No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGCCAAAATCTCGTATGCCGT3810.15419357971929482No Hit
CCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG3590.14529001343629092No Hit
ATTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT3550.1436711832030175Illumina PCR Primer Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATCTCGTATGCCGTCT3220.13031583377851166TruSeq Adapter, Index 4 (95% over 21bp)
CTTATACACATCTCCGAGCCAACGAGACCGGCCAATATCTCGTATGCCGT2870.11615106923736909TruSeq Adapter, Index 6 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCGG207.9198384E-569.9937350
ACCGAGA252.835901E-669.9937313
TTCGGCT207.9198384E-569.9937352
ATGCCGA650.069.9937344
CGTCAAC150.002223622369.9937348
AGGGGGG113600.065.095470
CGACTTC650.064.609648
ACGGCCA550.063.64354328
TAACTCG1000.063.01986336
GAACGGC501.4551915E-1163.00710726
AACTCGT850.061.7841837
ACTCGTA800.061.2693138
ATATCCG800.061.2445110
ACTCGAA352.9598777E-760.0189238
AACTCGA352.9598777E-760.0189237
TGCCGAC700.059.9946245
AGAACGG650.059.23744625
AAGGGGG127200.058.13551369
AACGGCC553.6379788E-1157.2791927
GCCAAAA1650.057.27918631
Produced by FastQC (version 0.11.5)