FastQCFastQC Report
Thu 26 May 2016
SRR1781454_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781454_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319644
Sequences flagged as poor quality0
Sequence length76
%GC76

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15554348.66132322208457No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGG150.002217938270.04362556
CGACGGC252.3866106E-456.00853322
CGACGAA252.3866106E-456.00853322
ACGCGGC200.006934659652.5244715
AGTGCGT200.00694757852.4997638
TCATATC200.00694757852.49976347
CGCCGTA604.9076334E-946.681153
GCGCGTG305.8821944E-446.651836
CCGACGG404.5831795E-543.75666421
GTGGTCG901.8189894E-1242.7842948
CGTATCA757.4942363E-1042.02617356
TGGTCGC757.530616E-1042.006449
TGCCGGC602.585657E-740.84596319
GCCGTAT701.9155777E-840.01236754
CGCGTCT350.001255400539.993557
CGTCTGG556.6770062E-638.1876379
CGGTGGT1200.037.91055346
ATCATTA806.192931E-835.0218159
GACGCGG400.00241130135.01631514
TCTGGCG806.2014806E-835.01631511
Produced by FastQC (version 0.11.5)