FastQCFastQC Report
Thu 26 May 2016
SRR1781454_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781454_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319644
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT11834937.02525309406715No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG114693.5880542103089685No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC58551.8317252943900089No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC27510.8606449675263731No Hit
ATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTCTT21450.6710590531966814RNA PCR Primer, Index 37 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC18560.5806459686401122No Hit
CTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG10670.3338088623593748No Hit
TTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC7850.24558571410694396No Hit
CTTTACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC7500.23463603258625218No Hit
TATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTCT5580.17456920824417166RNA PCR Primer, Index 37 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG5370.1679993993317566No Hit
CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGACGT5210.1629938306365832No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC5070.1586139580283065No Hit
CCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG4660.14578718824692471No Hit
CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCAGT4570.1429715558558897No Hit
ATTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT4040.12639060955312786No Hit
CTTATACACATCTCCGAGCCAACGAGACATGGGAACATCTCGTATGCCGT3870.12107219281450614No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGGGAACATCTCGTATGCCGT3830.1198208006407128No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACCG150.002220573170.022738
TAACGTA207.907166E-570.022738
CGAAAGC150.002221952270.01173441
CGAATGA301.0207259E-770.01173441
TAAGACG301.0207259E-770.01173443
TGACGTA207.919435E-570.0007845
GCCGTAA207.919435E-570.0007846
ATCAACG150.002224712269.9898310
TCAACGA150.002224712269.9898311
AGGGGGG137150.066.2390170
ATCGTAT800.065.6462839
AACTCGT700.065.0210737
AATGCCG700.065.01089543
TCGAATG1100.063.65699440
CAACTCG1000.063.0204236
TCTCGAA950.062.65188238
ACGTATG950.062.65188240
CGACTTC453.8380676E-1062.2229248
ATCTCGA850.061.7847337
ATATCGT850.061.7847337
Produced by FastQC (version 0.11.5)