FastQCFastQC Report
Thu 26 May 2016
SRR1781453_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781453_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225614
Sequences flagged as poor quality0
Sequence length76
%GC86

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15709269.62865779605876No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGGA252.8227041E-670.0453153
GTGGTCC150.002220787370.01420612
GTCGGGC150.002220787370.01420610
GTGCCGA150.002220787370.01420618
ACGTCTG150.00222470269.983148
TACATTC150.002226661269.96760624
ACGACCC207.932981E-569.96760624
CGGTGTA207.9417056E-569.9520934
CCTATCG207.9417056E-569.9520929
TGATGCG150.002228621969.9520931
GTGCCCT150.002228621969.9520942
CTAAGAG150.002230583769.936582
TGGGCGC352.956458E-760.02550549
GCTGCGG308.348272E-658.34517317
TGTGGAT252.384505E-456.01136816
CGACCCT252.3949906E-455.96166625
TCGGTGT252.3949906E-455.96166633
TGGCGCT900.054.45549813
GCGCTGC653.6379788E-1253.85708215
CACGTCT200.00695144352.487357
Produced by FastQC (version 0.11.5)