FastQCFastQC Report
Thu 26 May 2016
SRR1781453_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781453_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225614
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT11547451.18210749332931No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG117025.186734865744147No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC54202.402333188543264No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC26621.1798913188011382No Hit
ATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT25251.1191681367291035TruSeq Adapter, Index 7 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC17260.7650234471265082No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCGACCATCTCGTATGCCGT10930.48445575185937045No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACAATCTCGTATGCCGT10870.4817963424255587No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG10000.4432349056352886No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCGT9540.42284609997606537No Hit
TTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC8090.35857703865894847No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC7310.32400471601939596No Hit
TATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCT6090.26993005753189075No Hit
CTTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG5650.25042772168393806No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGACGT5280.2340280301754324No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC4880.21629863395002083No Hit
CCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG4290.1901477745175388No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT4220.1870451301780918No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCAGT3680.16311044527378618No Hit
CTTATACACATCTCCGAGCCAACGAGACCATCGACCATCTCGTATGCCGT3140.13917576036948062No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGAACATCTCGTATGCCGT2790.12366253867224551No Hit
CTTAAACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT2670.11834371980462205No Hit
TCTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT2380.10548990754119868No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAGAC150.002220096370.0197453
GACGTCA150.002222049770.0042146
GCAGTAT207.9124715E-570.0042146
AGTATTC207.9124715E-570.0042148
TAGCAAC150.002222049770.0042159
ATATCGA207.9124715E-570.0042137
GCCGAAT207.9124715E-570.0042146
GTCAACA150.002222049770.0042149
ACTCGTA750.070.0042138
CCGACTT455.456968E-1270.0042147
ACGTCAT150.002222049770.0042147
CGACTTC401.3460522E-1070.0042148
CACGTAT700.070.0042139
CGTCAAC150.002222049770.0042148
CGCATCA150.002224004669.988722
GACAACG150.002224004669.988718
AAGCCCG150.002224004669.988717
TACGAGA150.002224004669.988713
CTACGAC150.002224004669.988712
CCCGCAT150.002224004669.988720
Produced by FastQC (version 0.11.5)