FastQCFastQC Report
Thu 26 May 2016
SRR1781452_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781452_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302739
Sequences flagged as poor quality0
Sequence length76
%GC85

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20147966.55204648228342No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3060.10107716547917514No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG150.002221417870.0150652
CGGGTCT150.002225794869.9803167
TGTTAAT150.002227255469.9687530
TGCGGGT252.8434606E-669.968745
TTAGGCT150.002228716469.95718437
ATGTCGG150.00223164169.9340642
TATACGC150.002233104369.922513
TGTACGG150.002233104369.922513
TTATACG150.002234568169.910952
CCGACGG352.9709736E-759.99305321
CGTAGCA252.3798257E-456.03985656
CCGTAGC252.3817734E-456.03058255
TGTGGCG509.786163E-1056.00277711
TCGGGCG252.3876238E-456.00277711
GACGACA252.3895765E-455.99351523
GTCGGGC252.3895765E-455.99351510
CGCATCT252.3915306E-455.9842537
ACGAACC252.3915306E-455.98425324
CGTATGC252.3954423E-455.965741
TGTGCGG252.4013195E-455.9380043
Produced by FastQC (version 0.11.5)