FastQCFastQC Report
Thu 26 May 2016
SRR1781452_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781452_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302739
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT15748452.019726563145156No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG147714.879120298342797No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC71002.3452544931442594No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC27580.9110157594495589No Hit
ATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTT19610.6477526846557595RNA PCR Primer, Index 36 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC17190.5678158413683074No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACAATCTCGTATGCCGT15620.5159559884917372No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG10830.357733889588061No Hit
CTTTACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC7960.2629327572595536No Hit
TTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC7900.2609508520540796No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGACGT6690.22098243041035348No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG6060.20017242575287625No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT5980.19752988547891087No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCAGT5550.18332623150634705No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG5410.17870178602690767No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC5360.1770501983556793No Hit
TATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCT4800.15855241643792178No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT3830.12651161561609175No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACATCTCGTATGCCGTC3540.1169324071229673No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAAACATCTCGTATGCCGT3260.10768351616408853No Hit
CTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT3100.10239843561615783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG252.8353406E-670.0024836
CCGACTA252.8353406E-670.0024847
CGACTAC252.8353406E-670.0024848
ACGGGGG150.002223004870.0024770
TATCGAA150.002223004870.0024738
ACGCTAA550.069.9909227
GACGCTA550.069.9909226
GACAACG150.002224461969.9909118
ACGGGAA150.002224461969.9909127
GACGGGA150.002224461969.9909126
TACTCTA150.002224461969.9909131
ACCCACG207.924291E-569.9909118
ACGGTAG150.002224461969.9909127
AGGGGGG180750.067.1946470
ACCGAGC1200.064.1583413
GAGACGC600.064.1583424
TCACCGA1100.063.6281111
ATCACCG1100.063.6281110
CCGAGAC501.4551915E-1162.99182514
TAAGCCG950.062.63379743
Produced by FastQC (version 0.11.5)