FastQCFastQC Report
Thu 26 May 2016
SRR1781451_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781451_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347819
Sequences flagged as poor quality0
Sequence length76
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19354155.64417124998921No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCC150.002223974869.9969724
TCGGCAG150.00222651669.9768331
GATGTCG207.94516E-569.95669641
TATACGC150.002231604769.936563
TTGTGCG207.962164E-569.9265062
CGTATCA900.066.13682656
TCGCCGT950.062.63789752
GTATCAT1150.060.89323457
CGCCGTA1000.059.5145753
TGGTCGC1600.056.87254349
GTCGCCG1300.056.5360251
CCGTATC1000.056.0137155
GTATGCG252.4011392E-455.9412082
CGATGTG452.703382E-854.42642632
TTCGATG452.703382E-854.42642630
GTGGTCG2000.054.24765448
CGACGAA653.6379788E-1253.8438322
ATCTCGG1900.053.3803242
GCCGTAT1050.053.3463954
CGGTGGT2300.053.25856846