FastQCFastQC Report
Thu 26 May 2016
SRR1781451_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781451_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347819
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT14911242.87057348793481No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG150294.320925538857854No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC68951.9823528904401428No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC31410.9030558997639576No Hit
ATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTT25710.7391775607428002RNA PCR Primer, Index 36 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC20600.5922620673396221No Hit
CTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG13480.387557896492141No Hit
CTTTACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC8970.25789275456487426RNA PCR Primer, Index 36 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC8180.23517979178825768No Hit
CTTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG6650.1911913955246838No Hit
CCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG6100.1753785733384318No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC5770.16589088002668054No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGACGT5460.15697819843079303No Hit
TATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCT5420.1558281749990656RNA PCR Primer, Index 36 (95% over 22bp)
ATTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT5270.15151558713008778No Hit
CTTATACACATCTCCGAGCCAACGAGACTAACAACAATCTCGTATGCCGT5140.14777801097697366No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCAGT5080.1460529758293825No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATATCGTATGCCGT4530.1302401536431305No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACATCTCGTATGCCGTC4460.12822761263760749No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACAACAATCTCGTATGCCGT3620.10407712057133164No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAA301.0198346E-770.023339
ATATCGA150.00222066370.023337
AACTCGA150.00222066370.023337
CGTAAGA207.913446E-570.0132341
ACGATAC150.00222446669.99309522
TTGACGA150.00222446669.99309560
AGGGGGG174100.066.5758670
CCGTCTA1050.063.33619347
AAACTCG1500.063.0209736
ATGCCGA900.062.22503744
TGCCGAC900.062.22503745
CGTCTTA850.061.75861748
TCCGAGA1550.060.9617313
AACTCGT1400.060.0199737
CCGACTT700.060.0027147
CGACTTC700.059.99408748
ACCACGA1350.059.6237519
AAGGGGG195250.059.50758469
AACTCCG650.059.22492610
ACTCCGA650.059.22492611
Produced by FastQC (version 0.11.5)