FastQCFastQC Report
Thu 26 May 2016
SRR1781450_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781450_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305971
Sequences flagged as poor quality0
Sequence length76
%GC76

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15381350.27045046752797No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGG150.002224245369.99280521
CGTGTTA150.002225688469.9813729
TTCGATG150.00222713269.96992530
CGGTGTA252.8432569E-669.9699234
TCGCACT150.002228576569.958493
CGTATCA1000.063.04506356
CCGTATC800.061.28378755
ACGACAT252.3913984E-455.9850924
TCGATGT252.3933312E-455.97593731
TGTCGGG252.3933312E-455.97593743
CGCCGTA900.054.44775453
TCGCCGT850.053.5239152
AGCGGTA200.00692307752.54615859
GTATCAT1200.052.54615457
TAATTAG200.0069275652.5375561
GCGGTAT200.006932045352.5289654
GCGTATC200.006932045352.5289655
CGGCGTA200.00694551352.5031953
ATTCGAT200.006954502352.48602729
CATTCGA200.006954502352.48602728
Produced by FastQC (version 0.11.5)