SRR1781450_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1781450_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	305971
Sequences flagged as poor quality	0
Sequence length	76
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT	113952	37.242745227488875	Illumina PCR Primer Index 1 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG	11351	3.7098287092567594	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC	5251	1.7161757159992286	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC	2797	0.9141389216625104	No Hit
ATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCTT	2242	0.7327491821120302	Illumina PCR Primer Index 1 (95% over 24bp)
CTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC	1599	0.5225985469211134	Illumina PCR Primer Index 1 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACAACACGATCTCGTATGCCGT	1274	0.41637933006722855	TruSeq Adapter, Index 1 (95% over 23bp)
CTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG	992	0.3242137326740116	No Hit
CTTTACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC	741	0.24217981442685743	Illumina PCR Primer Index 1 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC	705	0.2304139934830425	Illumina PCR Primer Index 1 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCT	535	0.17485317235947198	Illumina PCR Primer Index 1 (95% over 23bp)
CTTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG	533	0.17419951564037114	No Hit
ATTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT	441	0.14413130656173298	Illumina PCR Primer Index 1 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC	434	0.14184350804488005	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAACACGATCTCGTATGCCGT	433	0.14151667968532966	No Hit
CCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG	429	0.140209366247128	No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATATCGTATGCCGT	426	0.13922888116847676	No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGACGT	386	0.12615574678646016	No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCAGT	381	0.12452160498870807	No Hit
CTTATACACATCTCCGAGCCCACGAGACCCACCACGATCTCGTATGCCGT	314	0.10262410489883028	Illumina PCR Primer Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCAACGAGACCCAACACGATCTCGTATGCCGT	309	0.10098996310107822	TruSeq Adapter, Index 1 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATCGT	45	5.456968E-12	69.99886	37
CGCGAGG	15	0.0022234842	69.998856	45
AACGAGC	15	0.0022234842	69.998856	13
CACGTAA	15	0.0022234842	69.998856	39
CGCGTAT	15	0.0022234842	69.998856	39
ATCGCGT	15	0.0022234842	69.998856	37
AGGGGGG	13670	0.0	66.209595	70
TACGAGC	80	0.0	65.623924	13
CACGAAC	110	0.0	63.635323	33
AATCGTA	50	1.4551915E-11	62.998974	38
ACGAACT	100	0.0	62.998974	34
TCGAAAG	40	1.063745E-8	61.248997	40
ACACGAA	175	0.0	59.99902	32
TATGACG	140	0.0	59.99902	43
CGAATGC	100	0.0	59.49903	41
ACGAAAT	65	0.0	59.229805	34
ACTCGTA	95	0.0	58.946407	38
AAGGGGG	15465	0.0	58.54738	69
GAAATCG	60	1.8189894E-12	58.33238	36
ACTCGAA	30	8.366274E-6	58.33238	38

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content