FastQCFastQC Report
Thu 26 May 2016
SRR1781449_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781449_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361606
Sequences flagged as poor quality0
Sequence length76
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20233655.95482375845533No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATC207.897325E-570.0427955
CGACGAA301.0238546E-769.9846422
GACGAAG150.002225602769.9846423
ACGAAGA150.002225602769.9846424
ACACGTC150.002228046869.965286
TACACGT150.002228046869.965285
TGGTCGG308.3794075E-658.32053849
GCGCGTC308.393166E-658.3043986
TATGCGC308.406942E-658.2882733
GCGGTAT252.3816322E-456.0342354
GTATGCG252.3947004E-455.9722252
GTCGGCG200.0069428252.5102851
TGACCCG200.006950425452.49574710
GACGAAC200.006954230452.48848323
TGCGGCA200.00695803752.4812226
CAGTGCG200.00695803752.4812228
CGATCTA200.00695803752.4812226
CGAAGAT200.00695803752.4812225
CGCGTCT407.495564E-752.4739657
CTAGGCG200.006961845352.4739654
Produced by FastQC (version 0.11.5)