FastQCFastQC Report
Thu 26 May 2016
SRR1781449_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781449_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361606
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT15360142.47744782995858No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG150104.150926699225123No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC68951.9067714584381896No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC30820.8523088665564178No Hit
ATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCTT23010.636327937036443TruSeq Adapter, Index 25 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC19490.5389844195063135No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCAGT12460.34457392852994695No Hit
CTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG11680.3230034899863387No Hit
TTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC8740.24169952932196923No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATCTCGTATGCCGTCT8740.24169952932196923No Hit
CTTTACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC8190.22648960470788648No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGACGT7470.20657843066763273No Hit
CCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG6550.1811363749495307No Hit
CTTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG6090.1684153470904797No Hit
ATTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT5680.15707703965089076No Hit
TATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCT4870.13467696885560526RNA PCR Primer, Index 25 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC4750.1313584398488963No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGAAATCTCGTATGCCGT4710.13025226351332667No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTAGTATGCCGT4360.12057322057709219No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTAAGCCGT4300.11891395607373773No Hit
CTTATACACATCTCCGAGCCCACGAGACATGAAGATATCTCGTATGCCGT4150.11476579481535151No Hit
CTTATACACATCTCCGAGCCAACGAGACATGATGATATCTCGTATGCCGT3880.10729910455025636No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGATGATATCTCGTATGCCGT3780.10453366371133221No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTG150.002221786470.0149434
AGCCGTA150.002223005870.0052645
TAAGACG150.002223005870.0052643
ATGTCCG150.002224225369.99557510
CCGTATA301.022745E-769.99557547
CGCTCGA150.002224225369.99557510
ACACTCG150.002224225369.99557527
AGGGGGG183650.066.5844170
ATGCCGA800.065.6299344
ACTCCGA750.065.329211
AATGCCG1050.063.3380943
CGAATGC1050.063.3380941
CTCGAAT1450.062.76333239
CGAGAAC453.8380676E-1062.2182915
TGCCGAC800.061.254645
TGACGTA352.9686817E-760.00450545
TCGTAAG1400.060.00450540
CGTAAGC1400.060.00450541
TCGAATG1400.060.00450540
CCGTAAT700.059.9962147
Produced by FastQC (version 0.11.5)