FastQCFastQC Report
Thu 26 May 2016
SRR1781448_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781448_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290912
Sequences flagged as poor quality0
Sequence length76
%GC85

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18982365.2510037399626No Hit
CTTATACACATCTGACGCTGCCGACGACCTTCACCGTGTAGATCTCGGTG3040.1044989550104499No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCA207.8757104E-570.0768856
CGGCGTA207.902651E-570.02859553
TCGGCGT252.8287723E-670.02859552
TGGCCGC150.002228740869.956296
ACGACAT150.002228740869.9562924
ACGAACG252.846189E-669.9562924
TAAACGT150.002230262669.9442537
TGTATAG150.002230262669.944255
CGTCATT150.002233308769.9201941
GGTCGGC352.9668627E-760.00383850
CGACGAG352.9739385E-759.9831822
TCAGTAA308.293155E-658.4175460
GCGTATC308.3101295E-658.39740455
GGCGTAT308.318626E-658.38733754
ATTCGAT308.403998E-658.28687729
TTATACG308.412575E-658.276852
CGACATT252.3974736E-455.955425
GTACGCG252.3974736E-455.95544
TTGTACG252.3995129E-455.9457782
CGTATCA1150.054.84277756
Produced by FastQC (version 0.11.5)