FastQCFastQC Report
Thu 26 May 2016
SRR1781448_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781448_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290912
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT14646850.34787152128478No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG145495.001168738312617No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC65002.234352656473435No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC28650.9848339016609834No Hit
ATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTT23900.8215542844571554Illumina PCR Primer Index 6 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC17910.6156500934990651No Hit
CTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG11570.39771477285227147No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAATCTCGTATGCCGTC9210.3165905840941591No Hit
TTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC8100.27843471565284345No Hit
CTTTACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC8090.2780909690903091No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCAAAAATCTCGTATGCCGT5900.20281047189528106No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGACGT5850.20109173908260916No Hit
CCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG5570.19146683533164668No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC5470.18802936970630293No Hit
CTTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG5470.18802936970630293No Hit
TATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCT5460.18768562314376855Illumina PCR Primer Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCAGT5140.17668573314266858No Hit
ATTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT5140.17668573314266858No Hit
CTTATACACATCTCCGAGCCAACGAGACGATCCAAAATCTCGTATGCCGT4870.16740457595424046No Hit
CTTAAACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT3730.12821746782532176No Hit
CTTATACACATCTCCGAGACCACGAGACGATCCAAAATCTCGTATGCCGT3380.11618633813661865No Hit
CTTATACACATCTCAGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT3110.10690518094819053No Hit
TCTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT3010.10346771532284678No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGT150.002222125270.00876637
TACTAGT150.002222125270.0087662
TGTACAC207.913768E-570.0087663
AATCGTA150.002222125270.00876638
TGCCGAC550.069.9967445
TCGCCGA150.00222364169.99673511
CTACTTG150.00222364169.99673514
TAGACGA150.00222364169.99673524
CACATCG150.00222364169.9967357
TGACGAC150.00222364169.99673545
CCGTATT455.456968E-1269.99673547
ACGTCTA252.8364448E-669.99673547
CGACTTA207.920511E-569.99673548
CGACTAC150.00222364169.99673548
ATGCCGA700.069.99673544
TAATGAT150.00222364169.99673552
ATCGCCG150.00222364169.99673510
GCCGTAT455.456968E-1269.99673546
TCGAAAG150.00222364169.99673540
TGCCGAA150.00222364169.99673545
Produced by FastQC (version 0.11.5)