FastQCFastQC Report
Thu 26 May 2016
SRR1781447_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781447_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248768
Sequences flagged as poor quality0
Sequence length76
%GC94

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20623282.90133779264214No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2940.11818240288139954No Hit
CTTATACACATCTGACGCTGCCGACGACCTTCACCGTGTAGATCTCGGTG2850.11456457422176484No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2550.10250514535631591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGC401.3460522E-1070.02235416
CGGATCA150.002220032570.02235456
CTGGCGA150.002220032570.02235418
GCGGCTG150.002221806470.0082558
ACGAGCT207.911781E-570.00825524
CGAGCTC207.911781E-570.00825525
GACGAGC207.911781E-570.00825523
GACGAAG150.002221806470.00825523
CGCGGCT150.002221806470.0082557
CGACGAA207.911781E-570.00825522
CCGACGG252.832452E-670.00825521
CGCGTCT150.002221806470.0082557
CGTCTGA150.002221806470.0082559
AATTAAC150.002225357669.9800834
CCTACCA150.00223069269.937852
TTGTACG150.00223069269.937852
TTGTGCG301.02729246E-769.937852
CATTAAA750.065.35419561
CGCCGTA600.064.1871653
CGTATCA550.063.65668556
Produced by FastQC (version 0.11.5)