FastQCFastQC Report
Thu 26 May 2016
SRR1781447_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781447_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248768
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT15354161.720558914329814No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG151316.08237393877026No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC77973.134245562130177No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC33821.3594996140982762No Hit
ATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCTT24770.9957068433239001Illumina PCR Primer Index 7 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC22170.8911917931566762No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG13180.5298109081553898No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCACGTATGCCGT10550.42408991510162075No Hit
TTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC9960.4003730383329045No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGACGT9800.3939413429379985No Hit
CTTTACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC8050.32359467455621305No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGACATCTCGTATGCCGT7070.2842005402624132No Hit
CTTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG6610.26570941600205816No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTAGTATGCCGT6530.2624935683046051No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC6490.2608856444558786No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTAAGCCGT6330.2544539490609725No Hit
CCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG6250.25123810136351943No Hit
TATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCT6160.24762027270388473No Hit
ATTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT5130.20621623359917676No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCAGT4890.1965686905068176No Hit
CTTATACACATCTCCGAGCCAACGAGACAAACGGTCATCTCGTATGCCGT4080.1640082325701055No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATATCGTATGCCGT3800.1527527656290198No Hit
TCTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT3070.12340815538976074No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCGGTCATCTCGTATGCCGT3050.12260419346539748No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGA2980.11979032673012607No Hit
CTTAAACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT2820.11335863133521996No Hit
CTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT2740.11014278363776693No Hit
CTTATACACATCTCAGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT2710.10893684075122202No Hit
CTTATACACATCTCCGAGACCACGAGACAAACGGTCATCTCGTATGCCGT2600.10451505016722408No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTAT150.002215060470.061971
CTAAAAC207.881793E-570.061971
CTTACTG150.002220369270.019713
ACACGTA207.913272E-570.0056338
GTATGGA150.00222214170.0056331
ACTGCAT207.913272E-570.0056353
AACGCAC150.00222214170.0056330
AACCGTC207.913272E-570.0056330
ACTCGAA207.913272E-570.0056338
GGGTCAT150.00222214170.0056332
GTGTATG150.00222214170.0056329
AACGAAC150.00222214170.0056330
CACGTAA401.3460522E-1070.0056339
TAACGAA150.00222214170.0056338
TCGAAAG353.6961865E-970.0056340
ATGAATG150.00222214170.0056354
ACCGTCA207.913272E-570.0056331
ACGTAAG353.6961865E-970.0056340
CATCATG150.00222214170.0056354
CACGAAT401.3460522E-1070.0056339
Produced by FastQC (version 0.11.5)