FastQCFastQC Report
Thu 26 May 2016
SRR1781446_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781446_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264281
Sequences flagged as poor quality0
Sequence length76
%GC91

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21630381.84583833117023No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG13420.5077928417101495No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150.002215680270.0582256
GCGGTGC150.002220685170.01839415
GTCTGGC252.8336963E-670.0051210
TGGCGGC207.914453E-570.0051219
CGCTGGC150.002222355270.0051216
GTGGCGC150.002222355270.0051212
CCATGTA150.002222355270.005129
TCTGGCG353.6961865E-970.0051211
GTCGACG150.002224026269.9918651
GGTCGAC150.002225698269.978650
CAAGGCC150.002225698269.978624
CCAAGGC150.002225698269.978623
GGTGTGC207.9442194E-569.9520951
TTATGCG150.002235750269.899152
CATACAG150.002235750269.899153
TGGGCGC150.002235750269.899153
TGGCGCT600.064.18352513
CTGCCGG550.063.64101418
TGCCGGC550.063.64101419
GGGTGCG352.9968578E-759.9135552
Produced by FastQC (version 0.11.5)