FastQCFastQC Report
Thu 26 May 2016
SRR1781446_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781446_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264281
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT17104864.72201936575085No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG120594.562946257960277No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC65122.464043953216463No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC22240.8415285245628706No Hit
ATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCTT15850.5997404278022257TruSeq Adapter, Index 11 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15090.5709831580779549No Hit
CTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC14890.5634154555189363No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATCTCGTATGCCGTCT11790.4461160658541477RNA PCR Primer, Index 38 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG11000.41622364074602414No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAGT10630.4022233910118396No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCCGT8430.3189786628626348No Hit
CTTTACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC7070.26751828546130824No Hit
ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT6100.23081492805006792No Hit
TTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC5720.21643629318793253No Hit
CTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT5020.18994933423136737No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG4770.1804897060325941No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG4760.18011132090464316No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTAAGCAGT4540.17178684808972267No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCAGT4480.1695165373220171No Hit
TATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCT4240.16043529425119474RNA PCR Primer, Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCAGCTAATCTCGTATGCCGTC4160.1574082132275873RNA PCR Primer, Index 29 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGACGT4080.15438113220397984No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTAAGCCGT3740.1415160378536482No Hit
CATATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT3620.13697541631823704No Hit
CTTAAACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT3500.13243479478282585No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC3230.12221839632815072No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTAAATCTCGTATGCCGT3220.12184001120019979No Hit
CTTATACACATCTCCGAGCCAACGAGACTCAGCTATATCTCGTATGCCGT3110.11767777479273955No Hit
TGACTTGCACATGTTGTTGTTGTTGTTTCTTTTGATCGGAAGAGCACACG2780.10519106557035883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTATG301.01974365E-770.0147640
ACTCGTA650.070.0147638
TAACGTA150.002221144770.0147638
TATAACG150.002221144770.0147636
ATAACGT150.002221144770.0147637
CTAGTGA353.6925485E-970.0147639
CGAGTGA150.002221144770.0147639
ATCACGA207.909069E-570.0147637
ACGAATG252.831388E-670.0147640
ATCGAGT207.909069E-570.0147637
TCGAGTG150.002221144770.0147638
ACCAGGC207.909069E-570.014761
GGCGCTG207.9164885E-570.001525
TGCCGAA207.9164885E-570.0015245
CCCGTCT207.9164885E-570.0015246
AGTGACC207.9164885E-570.0015241
CAGGCGC207.9164885E-570.001523
AATGCAG252.834573E-670.0015243
GACTCGG207.9164885E-570.0015226
CCAGGCG207.9164885E-570.001522
Produced by FastQC (version 0.11.5)