FastQCFastQC Report
Thu 26 May 2016
SRR1781445_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781445_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383281
Sequences flagged as poor quality0
Sequence length76
%GC81

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22828959.56178365220295No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG18110.4724992890333724No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATT207.92121E-570.00117518
CGAATTC150.002223618870.00117519
TCAACGC150.00222592569.982899
TTCGTAT150.002227078769.9737423
TGCGAGG150.002227078769.9737422
CGTATCG150.002228232869.964625
TATCGCA150.002228232869.964627
TGACGTA150.002231698369.937236
GACGTAC150.002232854369.9280737
TACGCGT150.002232854369.928075
ACGTACA150.002232854369.9280738
CGCGCAT150.002234010569.9189440
GTCGCGC252.4011836E-455.94245538
ATCATTA2750.053.5183959
CGCGAAT200.0069301152.53520617
CCCAACG200.0069301152.53520613
AACGCCC200.0069301152.53520611
GCCGTAT2800.052.52834354
TCGGCAT200.006951641352.49402248
TGGTCGC3000.052.4940249
Produced by FastQC (version 0.11.5)