FastQCFastQC Report
Thu 26 May 2016
SRR1781445_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781445_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383281
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT18743148.90171962607069No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG132683.4616899872417366No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC76581.9980119024945144No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC24120.6293033048859714No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21040.54894450807632No Hit
ATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCTT16670.4349289424730159TruSeq Adapter, Index 9 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC14570.3801388537391627No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG9560.24942535633125565No Hit
CTTTACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC6790.17715462023945877No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG5630.14688961884361604No Hit
TTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC5530.14428056699914685No Hit
ATTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT5500.14349785144580607No Hit
CTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT5090.13280073888348234No Hit
CATATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT4580.11949457447668942No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGATCTCGTATGCCGTC4490.11714642781666715RNA PCR Primer, Index 42 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG4350.11349375523441027No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAATCGGATCTCGTATGCCGT4340.11323285004996335No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT4140.10801474636102494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCACG401.3460522E-1070.018836
ACGGATA207.9113546E-570.018833
ACTCGTA600.070.0187938
ATATTCG150.002221402770.0187929
GTACGTC150.002221402770.0187934
CCGTCAT550.069.98225447
AGGGGGG209250.067.4246470
GTAATCG1800.066.1288729
TAATCGG1750.066.0177230
GACGTAA2000.064.7335926
GCCGTCA650.064.6074446
ATCGGAT1750.064.0171932
AAGGGGG223500.063.0944369
AATCGGA2000.063.0169231
ACGAATT950.062.6320527
CCGTCTA850.061.7490547
TGCCGTA401.0646545E-861.25045445
GACGAAT1200.061.2344726
ATATCGT1550.060.9841137
CGTAATC1900.060.79786328
Produced by FastQC (version 0.11.5)