FastQCFastQC Report
Thu 26 May 2016
SRR1781444_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781444_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450605
Sequences flagged as poor quality0
Sequence length76
%GC76

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24364754.07108221169317No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG20410.45294659402359044No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATC150.002226698269.978786
TGCGCGT207.948396E-569.9554755
GACGACA150.002229642869.9554735
CGCGTCT252.391738E-455.9892467
CTGGCGC653.6379788E-1253.85376412
GGACGCT200.006945419652.50741613
CGGCCGA200.00694847352.50158318
GTCGGGC200.00694847352.50158310
CGACGGC200.00695458252.48991822
CGATTAC200.00695458252.48991847
CCGACGG200.00695458252.48991821
GCACGTC200.006957637652.484096
TTATACG407.4954914E-752.478262
GGTTCGA200.006966811652.46660634
CGTATCA3150.051.1467556
TGGTCGC3300.050.91062549
CGCCGTA3100.050.83622753
CCGTATC3150.050.029355
GCCGTAT3200.049.24759754
ATCTCGG3350.049.07324242
Produced by FastQC (version 0.11.5)