FastQCFastQC Report
Thu 26 May 2016
SRR1781444_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781444_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450605
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT19872844.10248443758946No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG143553.1857169805039893No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC87081.9325129548052062No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC23530.5221868376959865No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23310.5173045128216509No Hit
ATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCTT18290.40589873614362915TruSeq Adapter, Index 2 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC12740.28273099499561705No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGATCTCGTATGCCGTC12120.26897171580430757No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG10340.22946926909377394No Hit
ATTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT6510.14447243150874936No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG6480.14380665993497632No Hit
CTTTACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC6090.13515162947592682No Hit
CTAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT5910.1311570000332886No Hit
CATATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT5180.1149565584048113No Hit
TTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC5130.11384693911518959No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATC301.02281774E-770.000566
GCGGAAT150.002225912469.9850224
CGACACG207.9317986E-569.98501623
AGGGGGG227500.067.2466870
GCCGTAT501.4551915E-1163.0074946
AAGGGGG244600.062.58839469
CCGTATT453.8380676E-1062.22962647
ACTCGTA850.061.77890438
TGACGTC1950.061.02612345
ACAACTC1550.060.961468
AATATCG1900.060.8034536
AAACTCG1050.060.01379836
CGCATCT352.9740113E-759.9938167
AACTCCG1050.059.9871610
AAAGGGG256400.059.74891368
AATCTCG251300.059.73517636
AAAAGGG257200.059.67193667
GAATCTC251650.059.66600835
TATGCCG251550.059.65527343
ATGCCGT251350.059.61918344