FastQCFastQC Report
Thu 26 May 2016
SRR1781443_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781443_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312856
Sequences flagged as poor quality0
Sequence length76
%GC88

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23900976.39584984785333No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG16780.5363489912291918No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGT150.002224634969.990087
AATTACC150.002226046869.9788844
CGTATCA2150.060.2529456
CCGTATC2250.057.5750355
GTGGTCG2500.057.40104748
TGGTCGC2350.056.60583549
CGCCGTA2300.056.32340253
GCCGTAT2300.056.32340254
TCGCCGT2250.056.01894852
AGTATGA252.3881186E-456.00102611
TAATGTC252.3881186E-456.00102622
TCGGTGG2750.055.98311245
TTGTGCG252.3956818E-455.9652062
TGTGCGG252.3956818E-455.9652063
CTATTAG252.3956818E-455.9652061
GGTCGCC2450.055.72422450
GGTGGTC2550.054.88540347
GTCGCCG2500.054.61847751
TCTCGGT2500.054.58353443
ATCTCGG2500.054.57480242
Produced by FastQC (version 0.11.5)