FastQCFastQC Report
Thu 26 May 2016
SRR1781443_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781443_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312856
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT19299861.6890837957399Illumina PCR Primer Index 6 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG133944.281202853709055No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC73762.3576341831385688No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC27390.8754826501649321No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19100.610504513258496No Hit
ATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCTT18960.6060296110670724Illumina PCR Primer Index 6 (95% over 24bp)
CTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC16190.5174904748510497Illumina PCR Primer Index 6 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG10440.33369984913186895No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATCTCGTATGCCGTCT8100.25890505536093283TruSeq Adapter, Index 4 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCCAATATCTCGTATGCCGT7790.24899634336563786TruSeq Adapter, Index 6 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC6550.20936149538445803Illumina PCR Primer Index 6 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC6300.20137059861405887Illumina PCR Primer Index 6 (95% over 22bp)
ATTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT6020.1924207942312118Illumina PCR Primer Index 6 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG5880.1879458920397883No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT5240.16748919630756642Illumina PCR Primer Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCAGT4900.15662157669982357No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGCCAAAATCTCGTATGCCGT4540.14511468535044877No Hit
CATATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT4500.1438361418671849Illumina PCR Primer Index 6 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG4400.14063978315902523No Hit
TATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCT4340.13872196793412944Illumina PCR Primer Index 6 (95% over 23bp)
CTTAAACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT4010.12817398419720255Illumina PCR Primer Index 6 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC3920.12529726135985886No Hit
CTTATACACATCTCCGAGCCAACGAGACCGGCCAATATCTCGTATGCCGT3920.12529726135985886TruSeq Adapter, Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGACGT3830.12242053852251517No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGCCAAATCTCGTATGCCGTC3790.12114199503925127No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGCAAATATCTCGTATGCCGT3530.11283146239803615No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG252.829047E-670.0295936
ACTCGAA252.829047E-670.0295938
CGTAAGA252.829047E-670.0295941
AACTCGA207.9030506E-570.0295937
CGAGTGA150.00221966370.0295839
TATATCG353.6889105E-970.0295836
ACGTAAG301.0188705E-770.0295840
CACGTAA301.0188705E-770.0295839
CGCCAAT150.00221966370.0295830
TAAGCCC207.915581E-570.00719543
ATGGGGG207.921853E-569.99670
ACTGCAT207.921853E-569.99653
CATATGC207.921853E-569.99651
TCACCGA1200.069.99611
ACGCATC252.8371196E-669.9966
GACCGTC150.002223890369.99626
GCTGCCT150.002223890369.99611
TGACGTA150.002223890369.99645
ATCACCG1200.069.99610
CCGTCCA150.002223890369.99628
Produced by FastQC (version 0.11.5)