FastQCFastQC Report
Thu 26 May 2016
SRR1781442_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781442_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507557
Sequences flagged as poor quality0
Sequence length76
%GC75

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25116649.48527948585085No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG19360.38143499153789623No Hit
AAAGAAACAACAACAACAACATGTGCAAGTCAAGATCGGAAGAGCGTCGT6840.1347631891590501No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGTT150.00221950270.0372155
GCACCGT150.002230818869.9474635
AACAACG509.786163E-1056.0131815
AAGATCG1050.053.2985632
CGTCGGG200.006951472352.496829
GGTATAC200.006975910652.4502563
CGGACTG200.006975910652.45025641
CAGATCG552.2955646E-950.87589332
AGATCGG2300.047.13850433
AACGGCC604.9294613E-946.66843818
CGTATCA2900.044.6789156
GATCGGA2450.044.25247634
CCGTATC2950.043.9216455
ACAACGG659.984433E-943.08706716
TCAAGAT1150.042.59350630
AGCGTCG2400.042.25575643
GAGCGTC2500.041.96434442
GCCGTAT3100.041.796454
GCGTCGT2350.041.67493444
CGTGTAG2200.041.3529748
Produced by FastQC (version 0.11.5)