FastQCFastQC Report
Thu 26 May 2016
SRR1781442_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781442_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507557
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT20548440.48491105432493No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG142352.804611107717951No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC91451.8017680772799902No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC27300.5378706233979632No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22560.44448209757721796No Hit
ATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTCTT20270.3993640123178283RNA PCR Primer, Index 37 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC15840.3120831748946424No Hit
CTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG11130.21928571569301575No Hit
CCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG7160.14106789976298229No Hit
CTTTACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC6750.1329899893016942No Hit
TGACTTGCACATGTTGTTGTTGTTGTTTCTTTAGATCGGAAGAGCACACG6620.13042870061884676No Hit
CTAATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT6160.12136567912569426No Hit
TTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC5980.11781927941098241No Hit
ATTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT5930.11683416837911802No Hit
CATATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT5120.1008753696629147No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATGC750.070.0138941
TCGTATC150.002222436570.0138940
CACGTAA252.8353606E-670.0138939
AAAATCG150.002222436570.0138934
ACCGGCA150.002225043769.993247
TACGAGA207.9281715E-569.993213
AGGGGGG232900.067.5438970
CGTTGTT501.4551915E-1163.00008812
TGGCCGT501.4551915E-1163.0000888
AAGGGGG251800.062.44627869
TTGGCCG453.8562575E-1062.2223057
ATCTACG1300.061.9231610
TCTACGA1300.061.9231611
CGTATGA1900.060.8015441
CCGTATT352.9746298E-759.9941747
AAAGGGG265250.059.25343368
AAAAAGG267000.059.08789466
AAAAGGG266400.059.05019467
AAAAAAG268550.058.9423365
GCTTGAA263600.058.86757356