FastQCFastQC Report
Thu 26 May 2016
SRR1781441_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781441_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387620
Sequences flagged as poor quality0
Sequence length76
%GC78

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22795058.807595067333985No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG17440.4499251844590062No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGG150.00222515469.9891522
ACATTCG150.002226294569.9801127
CGTCTGA150.002226294569.980119
CGACATT150.002226294569.9801125
ATTCGAT150.002227435469.9710729
GCATACG150.002228576769.96203644
GCGTATA150.00223314669.925921
CGTATAA150.002235433369.907872
CGCTGCG509.786163E-1056.00578716
TGCGGAC252.3878294E-456.00578719
CCGGCGA252.3908805E-455.99131822
GAGTATT252.3954631E-455.96962742
CGTATCA2700.054.4923256
CGTAGAT200.00692445652.54616561
GCCGTAT2800.052.52578754
CGCCGTA2800.052.51899753
TCGGATA200.0069457352.5054313
ACGACAT200.006956385452.4850824
TATGGAC200.006970611452.457983
CCGTATC2850.051.61095455
Produced by FastQC (version 0.11.5)