FastQCFastQC Report
Thu 26 May 2016
SRR1781441_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781441_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387620
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT18284747.171714565811875No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG129263.3347092513286207No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC71651.8484598317940253No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC25790.6653423455962024No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20200.5211289407151334No Hit
ATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT19000.4901707858211651TruSeq Adapter, Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCGT15090.3892987977916516No Hit
CTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC14920.3849130591816728No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCGACCATCTCGTATGCCGT10570.2726897476910376No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACAATCTCGTATGCCGT9890.2551467932511222No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG9630.24843919302409576No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC6440.16614209793096332No Hit
TTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC5940.15324286672514317No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT5840.15066302048397914No Hit
CCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG5660.14601929724988388No Hit
CTTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG4520.11660905010061401No Hit
TATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCT4490.1158350962282648No Hit
CTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT4380.11299726536298438No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC3970.10241989577421184No Hit
CATATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT3890.10035601878128064No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAATCG150.002220538770.0258236
CTCGAGC150.002220538770.0258240
CCTCGAC150.00222281370.00774429
GACGTCA252.837749E-669.9987146
ACTTATG207.9227204E-569.9987150
CGTCAAA207.9227204E-569.9987148
TGCCGAC455.456968E-1269.98968545
TCCGAAC150.002225089469.9896813
AGGGGGG211050.067.53564570
ATCACCG1000.066.490210
AACTCGT850.065.9066537
ATCTACG750.065.323710
AAGGGGG226300.062.9999969
ATGCCGA501.4551915E-1162.9988444
CAACTCG900.062.24516736
ACTCGTA850.061.78748338
CGACCAA850.061.77153432
ACCGAGC1250.061.59091613
ACGACCA401.063745E-861.25677531
TCACCGA1100.060.4456311
Produced by FastQC (version 0.11.5)