FastQCFastQC Report
Thu 26 May 2016
SRR1781440_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781440_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419923
Sequences flagged as poor quality0
Sequence length76
%GC77

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23027654.837672620932885No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG20820.49580518333122975No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAT150.00222128870.02087413
ATAGTCG150.00222128870.02087410
TAGTCCG150.002227669.97079531
TTCGTCG150.002228653369.96245643
CCTACGG200.006940783552.51565613
TAGTCGC200.006940783552.51565611
TTCACGG200.006947334452.5031324
CGCCGTA4300.051.3004853
CGTATCA4550.050.7904356
CCGTATC4450.050.35809355
GCCGTAT4450.050.35809354
ATCTCGG4350.049.85236442
TCTCGGT4450.049.52398743
TCGCCGT4550.049.2454552
GTGGTCG4950.048.79660448
GTCGCCG4600.048.71017551
TGGTCGC4600.048.7043649
TAGATCT4850.048.3130739
GGTCGCC4650.048.1806650
CGGTGGT4900.047.14004546
Produced by FastQC (version 0.11.5)