FastQCFastQC Report
Thu 26 May 2016
SRR1781440_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781440_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419923
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT18771244.70152861357916No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG134383.200110496448158No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC89612.1339626550581894No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24050.5727240470276693No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC23240.5534347963793362No Hit
ATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTT16740.398644513398885RNA PCR Primer, Index 36 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC12380.29481595435353625No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACAATCTCGTATGCCGT11340.27004950907666403No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG9040.21527756279127366No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT6350.15121819952705615No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG6230.14836053276434014No Hit
CTTTACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC5570.132643365569402No Hit
CTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT5350.12740430983775597No Hit
TTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC4850.11549736499310587No Hit
CATATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT4780.11383039271485487No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACATCTCGTATGCCGTC4520.10763878139563683No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGG150.002221592770.0184636
AAGTTCG150.002221592770.0184638
CATAACG301.02083504E-770.0184636
TAGCGCA150.002224743469.9934549
CGACTAT150.002224743469.99345425
AGGGGGG210400.068.1305170
AAGGGGG225850.063.42333669
CATCACG501.4551915E-1163.0166236
TAACGTA352.9659714E-760.01582738
AAAGGGG239500.059.85243668
AAAAGGG240650.059.6682267
CCGTCTT235250.059.6394847
GCCGTCT235850.059.57679446
TATGCCG236600.059.5577343
CGTCTTC235800.059.54488848
TGCCGTC236050.059.54113845
AAAAAGG241700.059.52483766
AAAAAAG242850.059.51677365
ATGCCGT236550.059.51113544
GTATGCC237050.059.4960542