FastQCFastQC Report
Thu 26 May 2016
SRR1781439_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781439_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282075
Sequences flagged as poor quality0
Sequence length76
%GC96

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24716787.6245679340601No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG21860.774971195604006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC150.002215696570.0593355
TGTGACG150.00221882170.03445411
CGGACGC150.00221882170.03445412
GCGCTGG353.6852725E-970.03445415
TGACGGT150.00221882170.03445413
GGACGCT150.00221882170.03445413
CGCTGGC252.8301329E-670.0220216
CGCTGCG207.905922E-570.0220216
CGCTGCA150.002220384570.0220216
GGCGTAT150.002220384570.0220254
GGGTGCC150.002220384570.0220216
GTGTGAC150.002220384570.0220210
TGCCGAA150.00222194970.0095919
TCGGCGT150.00222194970.0095952
GTCGGCG150.00222194970.0095951
GGTGTGA150.00222351469.997169
CGTCTGG207.9198406E-569.9971549
TTGAGTG150.002229782869.947532
CGGATGT150.002229782869.94757
GTAGGTC150.002229782869.947538
Produced by FastQC (version 0.11.5)