FastQCFastQC Report
Thu 26 May 2016
SRR1781439_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781439_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282075
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT20366872.20349197908358No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG143455.085526898874413No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC75332.670566338739697No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC27370.9703093148985199No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25180.8926703890809182No Hit
ATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTT19010.673934237348223RNA PCR Primer, Index 36 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC16240.5757334042364619No Hit
CTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG10710.37968625365594255No Hit
CTTTACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC7680.2722680138261101RNA PCR Primer, Index 36 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT6540.23185323052379686No Hit
ATTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT6440.22830807409376938No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACATCTCGTATGCCGTC5950.21093680758663474No Hit
CCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG5780.20491004165558804No Hit
CATATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT5670.20101036958255783No Hit
TTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC5640.1999468226535496No Hit
CTTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG4900.17371266507134628No Hit
TATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCT4500.15953203935123636RNA PCR Primer, Index 36 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT4180.14818753877514845No Hit
CTTATACACATCTCCGAGCCAACGAGACTAACAACAATCTCGTATGCCGT3950.14003367898608526No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC3900.13826110077107154No Hit
CTTATACACATCTCAGAGCCCACGAGACTAACAACAATCTCGTATGCCGT3600.1276256314809891No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGACGT3170.11238145883187096No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCAGT3040.10777275547283524No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATATCGTATGCCGT2930.10387308339980501No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGA207.90327E-570.0267937
TATCGAA207.90327E-570.0267938
AAAGTAT207.90327E-570.0267939
ATATCGA207.90327E-570.0267937
ACGAAAG207.90327E-570.0267940
AAGTATG252.828996E-670.0267840
ACACGAA301.0187796E-770.0267838
TCGAAAG500.070.0267840
CACGAAT252.828996E-670.0267839
CACGAAA150.002219788370.0267839
AATAGTA150.002219788370.0267838
CACCTAT150.002219788370.0267839
ATAGTAT301.0187796E-770.0267839
CACTCTA150.002219788370.0267835
TCACCTA150.002219788370.0267838
AAAACGT252.828996E-670.0267837
AATATAG150.002219788370.0267836
ACCTATG150.002219788370.0267840
AAACGTA252.828996E-670.0267838
TATAGTA150.002219788370.0267838
Produced by FastQC (version 0.11.5)