FastQCFastQC Report
Thu 26 May 2016
SRR1781438_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781438_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences313241
Sequences flagged as poor quality0
Sequence length76
%GC90

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25049779.96941651954884No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG15520.49546515302913735No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCA150.0022176370.0457656
GTCTCCA150.002220446270.0233515
TTCGTCT150.002220446270.0233551
CGTCTCC150.002220446270.0233514
TGACAGA150.002220446270.0233511
TCGTCTG150.002220446270.0233552
ATCTGGC150.002221855670.0121510
CGACGGG150.002223265470.0009622
TTGTTCG150.002223265470.0009648
CACGTCT207.925344E-569.989777
CGACCTG150.00222608769.9785846
TTAACGT150.002227498769.9673944
TAGTATC150.002228911369.95621532
CCAGGTC150.002230324369.945043
TCGACCT150.002230324369.945045
TTCGACC150.002230324369.945044
CTGCCGG650.059.2505318
TGCGGGT308.404946E-658.287535
GGTGTGC252.3957339E-455.9649731
TGCGCGT252.3976264E-455.9560285
Produced by FastQC (version 0.11.5)