FastQCFastQC Report
Thu 26 May 2016
SRR1781438_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781438_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences313241
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT20205064.50305036696984Illumina PCR Primer Index 1 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG139504.453440003064733No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC78262.498395803869864No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC27970.8929227016897533No Hit
ATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCTT19670.627951002582676Illumina PCR Primer Index 1 (95% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17540.5599522412455585No Hit
CTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC15520.49546515302913735Illumina PCR Primer Index 1 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACAACACGATCTCGTATGCCGT14150.45172886052592093TruSeq Adapter, Index 1 (95% over 23bp)
CTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG11620.3709603787499082No Hit
CTTTACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC7250.23145118295497719Illumina PCR Primer Index 1 (95% over 22bp)
ATTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT6430.2052732560552418Illumina PCR Primer Index 1 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC6200.197930666802877Illumina PCR Primer Index 1 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCAAACACGATCTCGTATGCCGT5770.18420321733106457No Hit
CCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG5450.17398744097994834No Hit
CTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT5290.16887955280439024Illumina PCR Primer Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCACCACGATCTCGTATGCCGT4890.15610983236549494Illumina PCR Primer Index 1 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG4850.1548328603216054No Hit
CATATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT4730.15100194418993682Illumina PCR Primer Index 1 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCT4160.13280509256451103Illumina PCR Primer Index 1 (95% over 23bp)
CTTAAACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT3950.126100989334091Illumina PCR Primer Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATATCGTATGCCGT3940.12578174632311864No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC3530.11269278287325095No Hit
CTTATACACATCTCCGAGCCAACGAGACCCAACACGATCTCGTATGCCGT3360.10726565168672046TruSeq Adapter, Index 1 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAA207.888531E-570.0556138
TATCACG150.002216396470.0556136
ATCACGA150.002216396470.0556137
ACTCGAA207.888531E-570.0556138
TATCGAA150.002216396470.0556138
TCACGTA700.070.0556138
TCTCGAA1000.070.055638
CACGAAT252.828181E-670.03322639
ATCGAAT150.002219207670.03322639
CAGCACG207.907282E-570.0220430
CGAAAGC401.3460522E-1070.01086441
ACGTAAG150.002222021370.01086440
TCGAAAG301.0207623E-770.01086440
GTTATAT150.002222021370.0108641
ACGAATG207.913541E-570.01086440
ATCCCGT207.919804E-569.9996944
TCCCGTC207.919804E-569.9996945
TATAGAC207.919804E-569.999693
TAAGACG207.919804E-569.9996943
TGATACA150.002223429369.999682
Produced by FastQC (version 0.11.5)