FastQCFastQC Report
Thu 26 May 2016
SRR1781437_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781437_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342121
Sequences flagged as poor quality0
Sequence length76
%GC83

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22453165.62911952204044No Hit
AAAGAAACAACAACAACAACATGTGCAAGTCAAGATCGGAAGAGCGTCGT21190.6193715089105901No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG20380.5956956749220305No Hit
AAAGAAACAACAACAACAACGGCCAAGGCCTCAGATCGGAAGAGCGTCGT7940.23208163193723855No Hit
AAAGAAACAACAACAACAACTATATTTTAAAGAGATCGGAAGAGCGTCGT5200.15199300832161722No Hit
AAAGAAACAACAACAACAACCCAGCGCCTGGTAGATCGGAAGAGCGTCGT3440.10054922088968522No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGAC150.00222649669.9767325
ACTCGTG150.00222908269.9562745
TATGCGC150.002232964869.9255753
ATCGGTG600.064.2393470
AAGATCG3500.062.96063232
CGACGAG401.0631993E-861.25653522
TCGGAAA352.988636E-759.9449736
CAACATG3450.059.87898617
AGTCAAG3450.059.82642428
TGGCGCT650.059.25443613
CAAGTCA3550.059.1266226
GTGCAAG3500.058.99765823
GTCAAGA3500.058.96313529
CAAGATC3750.058.76325631
GCAAGTC3400.058.65696725
TGCAAGT3350.058.4965724
ACAACAT3550.058.19225716
AAGTCAA3650.057.5067127
TGTGCAA3350.057.4688122
CTGCCGG553.6379788E-1157.28722418
Produced by FastQC (version 0.11.5)