FastQCFastQC Report
Thu 26 May 2016
SRR1781437_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781437_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342121
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT18117352.955825570485295No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG132433.870852710006109No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC80612.356183923231839No Hit
TGACTTGCACATGTTGTTGTTGTTGTTTCTTTAGATCGGAAGAGCACACG25300.7395044443340222No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC24040.7026753692407072No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23630.6906913051230413No Hit
ATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCTT16090.47030144305669636TruSeq Adapter, Index 25 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC11950.34929162489294724No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATCTCGTATGCCGTCT11420.3338000298140132No Hit
GAGGCCTTGGCCGTTGTTGTTGTTGTTTCTTTAGATCGGAAGAGCACACG9510.2779718286804961No Hit
CTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG8750.2557574659257982No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCAGT7460.21805150809216622No Hit
CTTTAAAATATAGTTGTTGTTGTTGTTTCTTTAGATCGGAAGAGCACACG6020.17596113655694914No Hit
ATTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT6000.17537654806340447No Hit
CCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG5470.1598849529844704No Hit
CTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT5100.1490700658538938No Hit
CTTTACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC4700.13737829598300016No Hit
TTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC4420.1291940570733746No Hit
CTTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG4270.12480964337178951No Hit
ACCAGGCGCTGGGTTGTTGTTGTTGTTTCTTTAGATCGGAAGAGCACACG4130.12071752391697674No Hit
CATATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT3980.11633311021539162No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGAATCTCGTATGCCGTC3570.10434904609772566No Hit
CTTAAACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT3550.10376445760418099No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTAT455.456968E-1270.0153539
ACGTATG500.070.0051240
ACACGAT150.002224209569.9948920
CGACATA150.002224209569.9948948
TTGCGCT150.002224209569.9948965
AACTCCG455.456968E-1269.9948910
ACTCCGA455.456968E-1269.9948911
AAAGCGG150.002224209569.9948968
ACGACTA301.022654E-769.9948947
CGACTAC252.83796E-669.9948848
CCGTTGT2300.068.4732511
ACTTGCA3750.068.148273
AGGGGGG205350.067.6600170
TTGGCCG2450.067.1379557
GCCGTTG2400.067.0784310
GACTTGC3800.066.330332
GTTTCTT10450.065.9760425
CGTTGTT2450.065.7094912
GGCCGTT2400.065.620219
TTGCACA3950.065.5845
Produced by FastQC (version 0.11.5)