FastQCFastQC Report
Thu 26 May 2016
SRR1781436_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781436_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410408
Sequences flagged as poor quality0
Sequence length76
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23103756.29446794409466No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG16700.406912145962067No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGT150.002219729470.03297452
GGTCGCA150.002221880570.0158750
CCGCTTG150.002221880570.0158714
CCTTACG150.002227265269.9731633
CATTCGA301.0253643E-769.9731628
CGACATT352.9791045E-759.9769925
CGACGAC650.059.22973622
GACGAAC308.377432E-658.325223
ATTCGAT308.389561E-658.31096329
TTATACG308.450428E-658.2399062
GGCCGAC252.3865401E-456.01269519
GACGACC252.3908628E-455.99218823
GACGACA509.804353E-1055.99218823
CGGGCTA252.3951917E-455.97169537
TACGCGT252.396636E-455.9648675
CGTATCA3450.053.80658356
GTATCAT3550.053.27752757
ATCATTA3600.052.54397659
GCCGGAT200.0069325852.53114354
ACTCCGT200.006952693752.49267623
Produced by FastQC (version 0.11.5)