FastQCFastQC Report
Thu 26 May 2016
SRR1781436_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781436_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410408
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT18896746.04369310539755No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG140613.4261028050135476No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC78291.9076138866688759No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC21120.5146098516598117No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19130.46612151809906244No Hit
ATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTT15120.3684138710746379Illumina PCR Primer Index 6 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC13110.3194382175786047No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAATCTCGTATGCCGTC12330.3004327401025321No Hit
CTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG8950.2180756710395509No Hit
ATTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT6370.15521139938792616No Hit
CCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG6100.14863258026159334No Hit
TTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC5610.1366932418471375No Hit
CTTTACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC5410.13182004249429835No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCAAAAATCTCGTATGCCGT5220.12719050310910118No Hit
CTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT5210.12694684314145924No Hit
CATATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT5040.12280462369154597No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTA150.002220658670.02560438
TCGTATC401.3460522E-1070.02560440
CGTATCC353.6980055E-970.0170741
TAGACGA252.841849E-669.9829424
AGGGGGG215550.067.7105170
ATCGTAT1500.067.69141439
TATCGTA1050.066.6910538
CGACTTC900.066.11111548
AAATCGT850.065.9064537
AATCGTA850.065.9064538
CGTATGA1150.063.92862741
ATATCGT1050.063.3564937
GCCGTCA1050.063.317946
AAGGGGG233850.062.40419869
CGATCAA2250.062.22221828
CCGTCAT900.062.2146447
TATCCGA900.062.20706611
ATCGTAA401.0617441E-861.272439
CGAAAGC401.0628355E-861.26493541
ATGCCGA1200.061.25000444
Produced by FastQC (version 0.11.5)