FastQCFastQC Report
Thu 26 May 2016
SRR1781435_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781435_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180910
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24264520.547289801932408No Hit
CTTATACACATCTGACGCTGCCGACGAAGATCTCTGTGTAGATCTCGGTG19270.1631792431260638No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGA150.002233079269.935423
CGTACGT200.0069320452.5383461
CGTATCA4450.037.77717256
CCGTATC4550.035.40144355
GCCGTAT4650.034.63718454
CGCCGTA4650.034.6327853
TCGCCGT4800.034.27550552
GTGGTCG6150.033.5718548
TGGTCGC5650.031.5891949
GTCGCCG5500.030.54443751
CGGTGGT6450.029.8363846
TCGGTGG6650.028.93291545
ATCTCGG5700.028.83546442
GTACGGA500.007231039527.9836465
GTATCAT6050.027.78651657
TCTCGGT6550.027.76532743
GATCTCG6150.026.72555441
CTCGGTG6700.026.62510344
ATCATTA6800.025.75299659
GGTCGCC6600.025.45154250
Produced by FastQC (version 0.11.5)