FastQCFastQC Report
Thu 26 May 2016
SRR1781435_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781435_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180910
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT19566116.568663149604966No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG136831.1586827107908308No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC88360.7482365294560974No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC25060.2122092284763445No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22870.1936642081106943No Hit
ATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCTT17480.14802144109203919Illumina PCR Primer Index 7 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC12750.10796758432056634No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG223650.067.2385870
AAGGGGG243250.061.7632569
CCGTCTT245700.059.2320347
CGTCTTC244400.059.2008748
GCCGTCT246450.059.12277646
ATGCCGT250050.059.0974344
TGCCGTC248000.059.06374745
TATGCCG250350.058.9846743
CGTATGC251200.058.80150641
TCGTATG251250.058.79229440
CTCGTAT251000.058.76717839
GTATGCC252100.058.64463842
TCTCGTA252050.058.591838
GCTTGAA251550.058.53632456
TGCTTGA248600.058.48474555
GTCTTCT245950.058.47202749
ATCTCGT253200.058.32568737
CTGCTTG247050.058.2849854
AAAGGGG258950.058.09966368
CATCTCG254250.058.09857636