FastQCFastQC Report
Thu 26 May 2016
SRR1781434_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781434_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406915
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19312647.46101765725029No Hit
CTTATACACATCTGACGCTGCCGACGATTCTAAGTGTGTAGATCTCGGTG11080.2722927392698721No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA2400.052.52668456
CGGTTAC200.006938243352.520229
AAGCCCG200.006938243352.5202217
CGCCGTA2250.051.35310453
TCGCCGT2250.051.34678352
CCGTATC2300.050.23673255
GTGGTCG2450.049.98848748
GTCGCCG2350.049.16181651
CGGTGGT2600.048.4384646
GGTCGCC2400.048.13760850
TGGTCGC2500.047.60075449
GCCGTAT2600.045.7868654
TCTCGGT2600.045.73618343
TCGGTGG2850.045.41695845
ATCTCGG2700.044.03683542
CTCGGTG2800.043.72916844
GATCTCG2750.043.2308541
GTATCAT2850.043.00959857
ATCATTA2950.042.74410259
GGTGGTC2900.042.2264647
Produced by FastQC (version 0.11.5)